Journal: Journal of Nanobiotechnology

Article Title: Targeting delivery of miR-146a via IMTP modified milk exosomes exerted cardioprotective effects by inhibiting NF-κB signaling pathway after myocardial ischemia-reperfusion injury

doi: 10.1186/s12951-024-02631-0

Figure Lengend Snippet: MEs-miR-146a protects H9c2 cells from OGD/R induced damage. ( A ) After 24 h of incubation, PKH26 labeled exosomes could be uptaken up by H9c2. Scale bar = 20 μm. ( B ) MEs-miR-146a decreased OGD/R induced cell apoptosis of H9c2 according to TUNEL assay. Scale bar = 50 μm. * P < 0.05, ** P < 0.01 versus the control group; & P < 0.05, && P < 0.01 versus the OGD/R group; ▲ P < 0.05 versus the OGD/R-MEs group. n = 6

Article Snippet: The one-step TUNEL Apoptosis Detection Kit (C1088, Green Fluorescence, Beyotime) was used to incubate for 60 min at RT, following by nuclear labeling with DAPI (H-1200-10, Vector Laboratories, Burlingame, CA, USA), both in the dark.

Techniques: Incubation, Labeling, TUNEL Assay, Control

Journal: Journal of Nanobiotechnology

Article Title: Targeting delivery of miR-146a via IMTP modified milk exosomes exerted cardioprotective effects by inhibiting NF-κB signaling pathway after myocardial ischemia-reperfusion injury

doi: 10.1186/s12951-024-02631-0

Figure Lengend Snippet: MEs-miR-146a protects NRCM cells from OGD/R induced damage. ( A ) Representative fluorescence images indicated that PKH26 labeled exosomes were taken up by NRCM cells after 24 h of incubation. Scale bar = 20 μm. ( B ) MEs-miR-146a decreased OGD/R induced cell apoptosis of NRCM according to TUNEL assay. Scale bar = 50 μm. ** P < 0.01 versus the control group; & P < 0.05, && P < 0.01 versus the OGD/R group; ▲ P < 0.05 versus the OGD/R-MEs group. n = 6

Article Snippet: The one-step TUNEL Apoptosis Detection Kit (C1088, Green Fluorescence, Beyotime) was used to incubate for 60 min at RT, following by nuclear labeling with DAPI (H-1200-10, Vector Laboratories, Burlingame, CA, USA), both in the dark.

Techniques: Fluorescence, Labeling, Incubation, TUNEL Assay, Control

Journal: Journal of Nanobiotechnology

Article Title: Targeting delivery of miR-146a via IMTP modified milk exosomes exerted cardioprotective effects by inhibiting NF-κB signaling pathway after myocardial ischemia-reperfusion injury

doi: 10.1186/s12951-024-02631-0

Figure Lengend Snippet: MEs-miR-146a exhibited better therapeutic efficacy in decreasing apoptotic cells and limiting inflammation than MEs. ( A - B ) Representative TUNEL staining images and quantification of apoptotic radio. ** P < 0.01 versus the Sham group; & P < 0.05 and && P < 0.01 versus the MIRI group. ( C - F ) The expression of inflammatory factors of rat serum were detected by ELISA. * P < 0.05, ** P < 0.01 versus the Sham group; & P < 0.05, && P < 0.01 versus the MIRI group; ▲ P < 0.05, ▲▲ P < 0.01 versus the MIRI-MEs-miR-146a group. n = 5

Article Snippet: The one-step TUNEL Apoptosis Detection Kit (C1088, Green Fluorescence, Beyotime) was used to incubate for 60 min at RT, following by nuclear labeling with DAPI (H-1200-10, Vector Laboratories, Burlingame, CA, USA), both in the dark.

Techniques: Drug discovery, TUNEL Assay, Staining, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Journal of Nanobiotechnology

Article Title: Targeting delivery of miR-146a via IMTP modified milk exosomes exerted cardioprotective effects by inhibiting NF-κB signaling pathway after myocardial ischemia-reperfusion injury

doi: 10.1186/s12951-024-02631-0

Figure Lengend Snippet: Tail vein injection of IMTP-MEs-miR-146a significantly ameliorated myocardium apoptosis and attenuated inflammation at the early stage of MIRI. ( A ) Representative H&E images of heart tissue from each group. Scale bar = 50 μm. ( B - C ) Representative images of TUNEL staining 24 h after MIRI and quantitative analysis of apoptotic radio. * P < 0.05 and ** P < 0.01 versus the MIRI group; & P < 0.05 versus the MIRI-MEs-miR-146a group. n = 8. ( D - G ) The expression of inflammatory factors of rat serum were detected by ELISA. ∆∆ P < 0.01 versus the MIRI group; ▲▲ P < 0.01 versus the MIRI-MEs-miR-146a group. n = 8

Article Snippet: The one-step TUNEL Apoptosis Detection Kit (C1088, Green Fluorescence, Beyotime) was used to incubate for 60 min at RT, following by nuclear labeling with DAPI (H-1200-10, Vector Laboratories, Burlingame, CA, USA), both in the dark.

Techniques: Injection, TUNEL Assay, Staining, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Immunology

Article Title: Neuroinflammatory and transcriptional dynamics during SARS-CoV-2 infection in KRT18-hACE2 mouse brain

doi: 10.3389/fimmu.2026.1716597

Figure Lengend Snippet: Neuropathological changes and inflammatory mediators in K18-hACE mouse brain following SARS-CoV-2 infection. (A, B) Immunofluorescence staining of brain tissue from uninfected and SARS-CoV-2 infected mice showing colocalization of DAPI (blue, nuclear stain) with (A) Iba-1 (green) and (B) CD68 (red). Lower panels show single staining for Iba-1 or CD68. Scale bar: 50 µm. (C) TUNEL assay of brain tissue from uninfected and SARS-CoV-2 infected mice. (D) Quantification of panel C showing the percentage of TUNEL+ cells in each group. Student t-test was performed to determine the significance. **p < 0.01, ***p < 0.001).

Article Snippet: DNA fragmentation was detected on rehydrated paraffin-embedded sections using a one-step TUNEL in situ apoptosis detection kit (E-CK-A325; Elabscience), according to the manufacturer’s instructions.

Techniques: Infection, Immunofluorescence, Staining, TUNEL Assay